Combination of small gap suture with nerve fragments in the experimental study of the rat nerve defects

Abstract

Objective: To study the effect of nerve fragments and small gap suture of epineurium in peripheral nerve repair.

Methods: (1) The experimental animals were randomly divided into control group, simple small gap suture group, small gap suture combined neural debris group; (2) observing the nerve anastomosis after two weeks; (3) testing nerve fiber regeneration after eight weeks; (4) testing gastrocnemius wet weight ratio after eight weeks; (5) pathological image analysis: the number of regenerated nerve fibers, the diameter and myelin thickness of regenerated nerve fiber were measured.

Results: (1) Scar tissue was formed in traditional suture anastomotic adhesions; in simple small gap suture group, obvious adhesions was not found surrounding the tissue; for combination group of small gap suture with nerve debris, there was no obvious adhesions, and the outer membrane of small gap turned thinner than before. (2) Compared with the normal control group, the wet weight was increased significantly in the small gap suture group; relative to the small gap group, the wet weight was increased significantly in the small gap plus nerve fragments group. (3) Compared with the control group, the total number of myelinated nerve fibers was increased significantly in the small-gap suture group and small gap plusnerve fragments group; the diameter of nerve fibers and myelin thickness were increased significantly in simple small gap group and the small gap plus nerve debris group. (4) Histology showed: Compared with simple small gap suture group, nerve fiber density was increased, arrangement is neat in the small gap plus nerve fragments group.

Conclusions: The nerve fragments plus epineurium small gap suture has a significant role in the repair of peripheral nerve injury.