Inhibition of ALA-PDT on A431 cells in cutaneous squamous cell carcinoma

Abstract

Objective: To determine the optimal dose of ALA-PDT on the inhibition of A431 cells proliferation in cutaneous squamous cell carcinoma.

Methods: A431 cells were cultured firstly in vitro, and then, were treated with different drug concentrations (0.5, 1 and 2 mmol/L) and different doses of PDT (5, 10, 20, 40 J/cm²). The proliferation of treated A431 cells was detected by MTT.

Results: The inhibition rates of ALA-PDT on A431 cells treated under the condition of 0.5 mmol/L ALA combined with 5, 10, 20 and 40 J/cm² PDT were 6%, 10%, 15% and 18% respectively. The inhibition rates of ALA-PDT on A431 cells treated with 1 mmol/L ALA combined with PDT of 5, 10, 20 and 40 J/cm² were 30%, 52%, 80% and 82% respectively. The inhibition rates of ALA-PDT on A431 cells treated with 2 mmol/L ALA combined with 5, 10, 20 and 40 J/cm² PDT were 31%, 54%, 81% and 82% respectively.

Conclusions: The highest inhibition rate of PDT on A431 cell proliferation can be acquired under the condition of 1 mmol/L ALA and 20 J/cm2 PDT.