Research of expression and correlation of ADAM8 and EGFR in the osteosarcoma tissue

Abstract

Objective: To lay a foundation for the research on the targeted therapy of osteosarcoma or the regulatory mechanism of the occurrence and development of osteosarcoma by studying the expression of a disintegrin and metalloproteinase domain-containing protein 8 (ADAM8) and epithelial growth factor receptor (EGFR) in osteosarcoma.

Methods: Osteosarcoma tissues, osteochondroma tissues, osteoarthritis tissues and normal bone tissues were selected and included in this research. By use of tissue chips and immunohistochemistry, the expression levels of ADAM8 and EGFR in the previously-mentioned tissues were observed and compared. Spearman’s rank correlation test was used to analyze the correlation of ADAM8 and EGFR and their relationship with clinicopathological factors in these cases.

Results: The positive expression levels of ADAM8 in osteosarcoma tissues, osteochondroma tissues, osteoarthritis tissues and normal bone tissues were 80.95%, 36.36%, 62.86% and 24.14% respectively, the difference was of statistical significance (p < .05); the positive expression levels of EGFR in osteosarcoma tissues, osteochondroma tissues, osteoarthritis tissues and normal bone tissues were 66.67%, 9.09%, 40.00% and 4.76% respectively, the difference was of statistical significance (p < .05); Spearman’s rank correlation test showed that ADAM8 was positively correlated with EGFR (r = .379, p < .05).

Conclusions: Both ADAM8 and EGFR have a much higher expression in osteosarcoma tissues, and the combined detection of two indexes can be considered as a new method in the clinical diagnosis of osteosarcoma.