Influence of thymopentin-5 on renal pathology and relevant indexes of serum in rats with acute kidney injury caused by sepsis

Abstract

Objective: To discuss thymopentin-5 on renal pathology and relevant indexes of serum with sepsis-caused acute kidney injury (AKI) caused by cecal ligation and puncture (CLP).

Methods: 90 cases of healthy and male SD rats were randomly divided into 3 groups: control group (C group), sepsis group (S group), thymopentin group (T group). These groups were divided into five time point including 1 h, 6 h, 12 h, 24 h and 48 h with 6 rats in each time point. The sepsis model was made by CLP. The blood and kidney tissue were collected in each time point. Changes of renal pathology were observed under light microscope and relevant indexes like serum creatinin (Cr), blood urea nitrogen (Bun), tumor necrosis factor α (TNF-α), interleukin-10 (IL-10), CD4+/CD8+ were tested and analyzed. 

Results: In T group, concentrations of Cr, Bun in 6 h after CLP started to rise, reached peak in 24 h and decreased in 48 h, which were all lower than S group (p < .05) and higher than C group (p < .05). Compared with C group, concentrations of TNF-α in 1 h significantly improved, reached peak in 12 h and decreased in 24 h, which were all lower than S group (p < .05); and concentrations of IL-10 in 1 h significantly decreased, reached peak in 12 h and rised in 24 h, which were all more than S group (p < .05). In T group, CD4+/CD8+ ratio in 6 h after CLP started to decrease, reached the lowest in 24 h and rised in 48 h, which were all lower than S group (p < .05) and significantly lower than C group (p < .05).

Conclusions: Thymopentin-5 plays the role of renal protection in AKI caused by sepsis.