Impacts of silencing CD105 and Ki67 gene expression on the biological behavior of ovarian cancer OVCAR3 cells

Abstract

Objective: To investigate the effect of silencing CD105 and Ki67 gene expression on OVCAR3 cell line in ovarian cancer.

Methods: According to cell transfection results, OVCAR3 cells were divided into CD105-siRNA, Ki67-siRNA, CD105-siRNA + Ki67-siRNA, negative control (NC1 and NC2) and control (non-transfection) groups. The effect of silencing CD105, Ki67 and their combined gene on proliferation, migration, invasion and apoptosis of human ovarian cancer cells can be studied and analyzed with MTT assay, wound-healing assay, Transwell chamber and flow cytometry.

Results: Compared with their respective negative control and liposome control groups, proliferation, migration and invasion abilities of OVCAR3 cells were decreased obviously in CD105-siRNA, Ki67-siRNA and CD105-siRNA + Ki67-siRNA groups after gene silencing. Whereas, the most significant change was detected in CD105-siRNA + Ki67-siRNA group along with obviously increased apoptosis rate of cancer cells, and the differences were of statistical significance (p < .01, p < .05).

Conclusions: Expression vectors of CD105-siRNA and Ki67-siRNA can inhibit the proliferation of OVCAR3 cells, reduce their abilities of migration and invasion, and induce apoptosis of tumor cells. The effect of combined gene silence will be more significant.