Expression and correlation of HSP70, Apaf-1 and ST13 in gastric polyps and gastric cancer

Abstract

Objective: To explore the expressions and relationship of HSP70, Apaf-1 and ST13 in the normal gastric mucosa, gastric polyps and gastric cancer, and to provide research basis and a new way for gastric cancer in the early diagnosis and clinical treatment.

Methods: Gastric mucosa samples of 72 patients who suffered from surgery in the Inner Mongolia Medical Baogang Hospital by gastroscopy from December 2014 to September 2015 were selected, and divided into normal gastric mucosa group, proliferative polyps group, adenomatous polyps group and adenocarcinoma cancer group. The levels of HSP70, Apaf-1 and ST13 proteins were detected by the Western blotting.

Results: The average IOD of HSP70 expressions was 0.33 ± 0.05, 0.46 ± 0.05, 0.77 ± 0.07, 0.93 ± 0.04 respectively in normal gastric mucosa group, proliferative polyps group, adenomatous polyps group and adenocarcinoma cancer group, the difference was statistically significant among four groups (p < .05). The average IOD of Apaf-1 expressions was 0.74 ± 0.03, 0.65 ± 0.03, 0.49 ± 0.06, 0.28 ± 0.05 respectively, the difference was statistically significant among four groups except for normal gastric mucosa and proliferative polyps (p < .05). The average IOD of ST13 expressions was 0.85 ± 0.11, 0.64 ± 0.07, 0.31 ± 0.06, 0.14 ± 0.01 respectively, the difference was statistically significant among four groups (p < .05). The expressions of HSP70 and Apaf-1 proteins were negatively correlated in the adenomatous polyps (r = -0.817, p < .05) and adenocarcinoma cancer (r = -0.816, p < .05). The expressions of HSP70 and ST13 proteins were negatively correlated in the adenomatous polyps (r = -0.818, p < .05) and adenocarcinoma cancer (r = -0.969, p < .05). The expressions of Apaf-1 and ST13 proteins were positively correlated in the adenomatous polyps (r = 0.830, p < .05) and adenocarcinoma cancer (r = 0.865, p < .05).

Conclusions: The expression of HSP70 increased progressively in the process of polyps canceration. It can be regarded as one of the indicators of early diagnosis of gastric cancer. The expressions of Apaf-1 and ST13 decreased progressively in the process of cancelation. The detection of the changes of Apaf-1 and decrease of ST13 specificity in gastric cancer can be learned deeply as diagnostic indicators for gastric cancer. During the canceration of gastric polyps, the expressions of HSP70 and Apaf-1, HSP70 and ST13 proteins were negatively correlated; Apaf-1 and ST13 protein were positively correlated. The joint detection of HSP70, Apaf-1 and ST13 may be helpful in early diagnosis of gastric cancer.