Expressions of miRNA29 target genes CCND2 and CDK6 in cervical cancer

Abstract

Objective: To study the expression of miRNA29 target genes recombinant cyclin D2 (CCND2) and cyclin-dependent kinase 6 (CDK6) in cervical squamous cell carcinoma tissues and their relationship with clinicopathological factors.

Methods: Levels of mRNA of CCND2 and CDK6 in cervical squamous cell carcinoma tissues, cervical intraepithelial neoplasia (CIN) tissues and normal cervical tissues were detected by reverse transcription-polymerase chain reaction (RT-PCR).

Results: There was a statistical difference in the expression of CCND2 (one of miRNA29 target genes) in normal cervical tissues, CIN tissues and cervical squamous cell carcinoma tissues (H = 29.27, p = .00), but there was no statistical difference in the expression of CDK6 (one of miRNA29 target genes) in them (H = 2.76, p = .25). CCND2 was positively correlated to CDK6 in CIN tissues (r = 0.58, p < .05).

Conclusions: CCND2, one of miRNA29 target genes, may be involved in the occurrence and development of cervical cancer, but CDK6 is less relevant to the occurrence and development of cervical cancer; CCND2 and CDK6 may play a synergistic role in the occurrence and development of CIN.